The Enzyme Function Initiative (EFI) is a Large‑Scale Collaborative Project (NIGMS U54GM09334205) that is developing tools and strategies to enable more accurate predictions of enzymatic activities and physiological functions of unknown/uncharacterized enzymes discovered in genome sequencing projects. The goal of this project is to calculate sequence similarity networks (SSNs) for the complete library of Pfamdefined protein families (>98,000,000 sequences, January 2015). SSNs allow experimentalists to visualize the relationship between protein sequence identity and enzymatic function for a specific protein family (hundreds to thousands of proteins). To date, we have been able to generate 97% of the Pfam (v48) library. We focused our efforts on developing, scaling and more efficiently running our production pipeline, and have developed strategies for effectively dealing with the ever increasing genomic dataset. Our current goal is to begin generating and disseminating complete libraries of SSNs to the biological community for 2015.
